Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-), SignalSilence ® IKKβ siRNA I (+) or SignalSilence ® IKKβ siRNA II #6378 (+), using IKKβ (2C8) Rabbit mAb #2370 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The IKKβ (2C8) Rabbit mAb confirms silencing of IKKβ expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.